Abstract
Caspase-1 is a critical factor in the innate immune response to Legionella pneumophila. The development of methods for analyzing caspase-1 activation pathways and downstream caspase-1-associated activities has helped in understanding the regulation of this protease and the signaling components involved. Here we outline methods for directly detecting active caspase-1, measuring caspase-1 activities and analyzing components involved in the regulation of caspase-1 during L. pneumophila infection in macrophages.
MeSH terms
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Animals
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Apoptosis Regulatory Proteins / metabolism
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Bone Marrow Cells / enzymology
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Bone Marrow Cells / microbiology
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CARD Signaling Adaptor Proteins
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Calcium-Binding Proteins / metabolism
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Caspase 1 / metabolism*
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Cytoskeletal Proteins / metabolism
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Enzyme Activation
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Legionella pneumophila / growth & development
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Legionella pneumophila / metabolism*
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Macrophages / metabolism*
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Macrophages / microbiology*
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Mice
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Neuronal Apoptosis-Inhibitory Protein / metabolism
Substances
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Apoptosis Regulatory Proteins
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CARD Signaling Adaptor Proteins
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Calcium-Binding Proteins
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Cytoskeletal Proteins
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Ipaf protein, mouse
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Naip5 protein, mouse
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Neuronal Apoptosis-Inhibitory Protein
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Pycard protein, mouse
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Caspase 1