Abstract
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Bone Neoplasms / enzymology
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Caenorhabditis elegans / cytology*
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Cell Tracking*
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Chromosomal Proteins, Non-Histone / metabolism
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DNA-Binding Proteins / metabolism
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Humans
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Imaging, Three-Dimensional / methods*
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Microscopy, Fluorescence*
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Neurons / cytology*
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Osteosarcoma / enzymology
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RNA Polymerase II / metabolism
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae Proteins / metabolism
Substances
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CSE4 protein, S cerevisiae
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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RNA Polymerase II