Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal

Takashi Yamano; Hiro Iguchi; Hideya Fukuzawa

doi:10.1016/j.jbiosc.2012.12.020

Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal

J Biosci Bioeng. 2013 Jun;115(6):691-4. doi: 10.1016/j.jbiosc.2012.12.020. Epub 2013 Jan 18.

Authors

Takashi Yamano¹, Hiro Iguchi, Hideya Fukuzawa

Affiliation

¹ Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.

PMID: 23333644
DOI: 10.1016/j.jbiosc.2012.12.020

Abstract

Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Wall
Chlamydomonas reinhardtii / genetics*
Electroporation / methods*
Transformation, Genetic*