Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer

A Ishchenko; E Round; V Borshchevskiy; S Grudinin; I Gushchin; J P Klare; T Balandin; A Remeeva; M Engelhard; G Büldt; V Gordeliy

doi:10.1016/j.jphotobiol.2013.03.008

Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer

J Photochem Photobiol B. 2013 Jun 5:123:55-8. doi: 10.1016/j.jphotobiol.2013.03.008. Epub 2013 Mar 29.

Authors

A Ishchenko¹, E Round, V Borshchevskiy, S Grudinin, I Gushchin, J P Klare, T Balandin, A Remeeva, M Engelhard, G Büldt, V Gordeliy

Affiliation

¹ Institute of Complex Systems (ICS), ICS-5: Molecular Biophysics, Research Centre Juelich, 52425 Juelich, Germany.

PMID: 23619282
DOI: 10.1016/j.jphotobiol.2013.03.008

Abstract

The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Archaeal Proteins / chemistry*
Archaeal Proteins / genetics
Archaeal Proteins / physiology
Archaeal Proteins / radiation effects
Carotenoids / chemistry*
Carotenoids / genetics
Carotenoids / radiation effects
Crystallography, X-Ray
Halobacteriaceae / chemistry
Halorhodopsins / chemistry*
Hydrogen Bonding
Intracellular Signaling Peptides and Proteins / chemistry*
Intracellular Signaling Peptides and Proteins / genetics
Light
Models, Molecular
Multiprotein Complexes / chemistry
Multiprotein Complexes / radiation effects
Rhodopsins, Microbial / chemistry*
Rhodopsins, Microbial / genetics
Sensory Rhodopsins / chemistry*
Signal Transduction

Substances

Archaeal Proteins
Halorhodopsins
HtrII protein, Natronobacterium pharaonis
Intracellular Signaling Peptides and Proteins
Multiprotein Complexes
Rhodopsins, Microbial
Sensory Rhodopsins
phototaxis receptor sensory rhodopsin II, Natronobacterium pharaonis
Carotenoids

Associated data

PDB/4GYC