Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae

Tohru Miyoshi-Akiyama; Kayoko Hayakawa; Norio Ohmagari; Masahiro Shimojima; Teruo Kirikae

doi:10.1371/journal.pone.0066358

Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae

PLoS One. 2013 Jun 11;8(6):e66358. doi: 10.1371/journal.pone.0066358. Print 2013.

Authors

Tohru Miyoshi-Akiyama¹, Kayoko Hayakawa, Norio Ohmagari, Masahiro Shimojima, Teruo Kirikae

Affiliation

¹ Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan.

Abstract

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enterobacter cloacae / genetics*
Multilocus Sequence Typing / methods*

Grants and funding

This study was supported by the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases, the Ministry of Education, Culture, Sports, Science and Technology, Japan (http://www.riken.jp/en/research/labs/crnid/). TMA was supported by a Grant for International Health Research (23A301) from the Ministry of Health, Labor, and Welfare, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.