Laser speckle contrast imaging: theoretical and practical limitations

J Biomed Opt. 2013 Jun;18(6):066018. doi: 10.1117/1.JBO.18.6.066018.

Abstract

When laser light illuminates a diffuse object, it produces a random interference effect known as a speckle pattern. If there is movement in the object, the speckles fluctuate in intensity. These fluctuations can provide information about the movement. A simple way of accessing this information is to image the speckle pattern with an exposure time longer than the shortest speckle fluctuation time scale-the fluctuations cause a blurring of the speckle, leading to a reduction in the local speckle contrast. Thus, velocity distributions are coded as speckle contrast variations. The same information can be obtained by using the Doppler effect, but producing a two-dimensional Doppler map requires either scanning of the laser beam or imaging with a high-speed camera: laser speckle contrast imaging (LSCI) avoids the need to scan and can be performed with a normal CCD- or CMOS-camera. LSCI is used primarily to map flow systems, especially blood flow. The development of LSCI is reviewed and its limitations and problems are investigated.

Publication types

  • Review

MeSH terms

  • Algorithms
  • Blood Flow Velocity
  • Contrast Media / pharmacology
  • Erythrocytes / pathology
  • Humans
  • Lasers*
  • Microcirculation / physiology*
  • Optics and Photonics
  • Perfusion
  • Regional Blood Flow
  • Retinal Vessels / pathology
  • Ultrasonography, Doppler / methods

Substances

  • Contrast Media