Regulated expression of PTPRJ/CD148 and an antisense long noncoding RNA in macrophages by proinflammatory stimuli

Richa K Dave; Marcel E Dinger; Megan Andrew; Marjan Askarian-Amiri; David A Hume; Stuart Kellie

doi:10.1371/journal.pone.0068306

Regulated expression of PTPRJ/CD148 and an antisense long noncoding RNA in macrophages by proinflammatory stimuli

PLoS One. 2013 Jun 28;8(6):e68306. doi: 10.1371/journal.pone.0068306. Print 2013.

Authors

Richa K Dave¹, Marcel E Dinger, Megan Andrew, Marjan Askarian-Amiri, David A Hume, Stuart Kellie

Affiliation

¹ The University of Queensland, Institute for Molecular Bioscience, Brisbane, Australia.

Abstract

PTPRJ/CD148 is a tyrosine phosphatase that has tumour suppressor-like activity. Quantitative PCR of various cells and tissues revealed that it is preferentially expressed in macrophage-enriched tissues. Within lymphoid tissues immunohistochemistry revealed that PTPRJ/CD148 co-localised with F4/80, indicating that macrophages most strongly express the protein. Macrophages express the highest basal level of ptprj, and this is elevated further by treatment with LPS and other Toll-like receptor ligands. In contrast, CSF-1 treatment reduced basal and stimulated Ptprj expression in human and mouse cells, and interferon also repressed Ptprj expression. We identified a 1006 nucleotide long noncoding RNA species, Ptprj-as1 that is transcribed antisense to Ptprj. Ptprj-as1 was highly expressed in macrophage-enriched tissue and was transiently induced by Toll-like receptor ligands with a similar time course to Ptprj. Finally, putative transcription factor binding sites in the promoter region of Ptprj were identified.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
HEK293 Cells
Humans
Inflammation / genetics*
Inflammation / metabolism
Macrophage Colony-Stimulating Factor / genetics
Macrophage Colony-Stimulating Factor / metabolism
Macrophages / metabolism*
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
NIH 3T3 Cells
Phagocytes / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Untranslated / genetics*
RNA, Untranslated / metabolism
Receptor-Like Protein Tyrosine Phosphatases, Class 3 / genetics*
Receptor-Like Protein Tyrosine Phosphatases, Class 3 / metabolism*
Toll-Like Receptors / genetics
Toll-Like Receptors / metabolism

Substances

RNA, Messenger
RNA, Untranslated
Toll-Like Receptors
Macrophage Colony-Stimulating Factor
Receptor-Like Protein Tyrosine Phosphatases, Class 3

Grants and funding

Part of this work was funded by the CRC for Chronic Inflammatory Diseases. RKD was in receipt of an NHMRC Dora Lush scholarship. MED is in receipt of an NHMRC Career Development Award. The authors wish to acknowledge the ARC Special Research Centre for Functional and Applied Genomics. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.