Abstract
Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Actin Cytoskeleton
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Biosensing Techniques
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Cell Line, Tumor
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Cell Movement / genetics*
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Cell Surface Extensions / genetics
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Extracellular Matrix / metabolism
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Extracellular Matrix / ultrastructure
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Humans
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Molecular Biology / methods*
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Molecular Imaging / methods*
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Neoplasms / pathology*
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rho GTP-Binding Proteins / chemistry
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rho GTP-Binding Proteins / metabolism*