Abstract
The phytohormone auxin is a key developmental signal in plants. So far, only auxin perception has been described to trigger the release of transcription factors termed Auxin Response Factors (ARFs) from their auxin/indole-3-acetic acid (AUX/IAA) repressor proteins. Here, we show that phosphorylation of ARF7 and ARF19 by BRASSINOSTEROID-insensitive2 (BIN2) can also potentiate auxin signalling output during lateral root organogenesis. BIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes lateral organ boundaries-domain16 (LBD16) and LBD29. In this context, BIN2 is under the control of the Tracheary element differentiation inhibitory factor (TDIF)-TDIF receptor (TDR) module. TDIF-initiated TDR signalling directly acts on BIN2-mediated ARF phosphorylation, leading to the regulation of auxin signalling during lateral root development. In summary, this study delineates a TDIF-TDR-BIN2 signalling cascade that controls regulation of ARF and AUX/IAA interaction independent of auxin perception during lateral root development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / drug effects
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Arabidopsis Proteins / pharmacology*
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DNA, Plant / metabolism
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Gene Expression Regulation, Plant / drug effects
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Indoleacetic Acids / pharmacology*
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Models, Biological
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Molecular Sequence Data
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Mutation / genetics
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Oligopeptides / pharmacology*
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Phosphorylation / drug effects
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Plant Roots / drug effects
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Plant Roots / genetics
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Plant Roots / growth & development*
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Protein Binding / drug effects
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Protein Binding / genetics
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Protein Kinases / metabolism*
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Signal Transduction
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic / drug effects
Substances
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ARF19 protein, Arabidopsis
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ARF7 protein, Arabidopsis
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Arabidopsis Proteins
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DNA, Plant
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Indoleacetic Acids
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Oligopeptides
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Transcription Factors
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tracheary element differentiation inhibitory factor, Arabidopsis
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Protein Kinases
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BIN2 protein, Arabidopsis
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PXY protein, Arabidopsis