Abstract
We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Adaptor Proteins, Vesicular Transport / metabolism
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Adaptor Proteins, Vesicular Transport / ultrastructure
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Clathrin / ultrastructure
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Gold / chemistry*
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Humans
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Membrane Proteins / metabolism
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Membrane Proteins / ultrastructure*
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Microscopy, Electron, Transmission*
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Microscopy, Fluorescence*
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Nanotubes / chemistry*
Substances
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Adaptor Proteins, Vesicular Transport
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Clathrin
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Membrane Proteins
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epsin
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Gold