Characterization of human mesenchymal stem cells from ewing sarcoma patients. Pathogenetic implications

PLoS One. 2014 Feb 3;9(2):e85814. doi: 10.1371/journal.pone.0085814. eCollection 2014.

Abstract

Background: Ewing Sarcoma (EWS) is a mesenchymal-derived tumor that generally arises in bone and soft tissue. Intensive research regarding the pathogenesis of EWS has been insufficient to pinpoint the early events of Ewing sarcomagenesis. However, the Mesenchymal Stem Cell (MSC) is currently accepted as the most probable cell of origin.

Materials and methods: In an initial study regarding a deep characterization of MSC obtained specifically from EWS patients (MSC-P), we compared them with MSC derived from healthy donors (MSC-HD) and EWS cell lines. We evaluated the presence of the EWS-FLI1 gene fusion and EWSR1 gene rearrangements in MSC-P. The presence of the EWS transcript was confirmed by q-RT-PCR. In order to determine early events possibly involved in malignant transformation, we used a multiparameter quantitative strategy that included both MSC immunophenotypic negative/positive markers, and EWS intrinsic phenotypical features. Markers CD105, CD90, CD34 and CD45 were confirmed in EWS samples.

Results: We determined that MSC-P lack the most prevalent gene fusion, EWSR1-FLI1 as well as EWSR1 gene rearrangements. Our study also revealed that MSC-P are more alike to MSC-HD than to EWS cells. Nonetheless, we also observed that EWS cells had a few overlapping features with MSC. As a relevant example, also MSC showed CD99 expression, hallmark of EWS diagnosis. However, we observed that, in contrast to EWS cells, MSC were not sensitive to the inhibition of CD99.

Conclusions: In conclusion, our results suggest that MSC from EWS patients behave like MSC-HD and are phenotypically different from EWS cells, thus raising important questions regarding MSC role in sarcomagenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 12E7 Antigen
  • Antigens, CD / metabolism
  • Antigens, CD34 / metabolism
  • Calmodulin-Binding Proteins / genetics*
  • Cell Adhesion Molecules / metabolism
  • Cell Line
  • Cells, Cultured
  • Endoglin
  • Flow Cytometry
  • Gene Expression Regulation, Neoplastic
  • Gene Rearrangement
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukocyte Common Antigens / metabolism
  • Mesenchymal Stem Cells / metabolism*
  • Mesenchymal Stem Cells / pathology
  • Oncogene Proteins, Fusion / genetics*
  • Proto-Oncogene Protein c-fli-1 / genetics*
  • RNA-Binding Protein EWS / genetics*
  • RNA-Binding Proteins / genetics*
  • Receptors, Cell Surface / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Ewing / genetics*
  • Sarcoma, Ewing / metabolism
  • Sarcoma, Ewing / pathology
  • Thy-1 Antigens / metabolism

Substances

  • 12E7 Antigen
  • Antigens, CD
  • Antigens, CD34
  • CD99 protein, human
  • Calmodulin-Binding Proteins
  • Cell Adhesion Molecules
  • ENG protein, human
  • EWS-FLI fusion protein
  • EWSR1 protein, human
  • Endoglin
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Protein c-fli-1
  • RNA-Binding Protein EWS
  • RNA-Binding Proteins
  • Receptors, Cell Surface
  • Thy-1 Antigens
  • Leukocyte Common Antigens
  • PTPRC protein, human

Grants and funding

Enrique de Álava's lab is also sourced by the Red Temática de investigación del cáncer (RTICC, Spain). Katia Scotlandi's lab is also sourced by the Italian Association for cancer research (AIRC IG10452). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.