Optimized ferrozine-based assay for dissolved iron

Thomas M Jeitner

doi:10.1016/j.ab.2014.02.026

Optimized ferrozine-based assay for dissolved iron

Anal Biochem. 2014 Jun 1:454:36-7. doi: 10.1016/j.ab.2014.02.026. Epub 2014 Mar 12.

Author

Thomas M Jeitner¹

Affiliation

¹ Neurosciences, Winthrop University Hospital, Suite 502, 222 Station Plaza North, Mineola, NY 11501, USA. Electronic address: Tjeitner@Winthrop.org.

PMID: 24632099
DOI: 10.1016/j.ab.2014.02.026

Abstract

The following report describes a simple and optimized assay for the detection of iron in solution based on the binding of this metal by ferrozine. This assay accurately measures between 1 and 200 μM sample iron concentrations within 2½ hours.

Keywords: Ferrozine; Iron.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Absorption, Physicochemical
Chemistry Techniques, Analytical / methods*
Ferrozine / chemistry*
Iron / analysis*
Iron / chemistry*
Limit of Detection

Substances

Ferrozine
Iron

Grants and funding

R03 NS074286/NS/NINDS NIH HHS/United States