Targeted genomic rearrangements using CRISPR/Cas technology

Peter S Choi; Matthew Meyerson

doi:10.1038/ncomms4728

Targeted genomic rearrangements using CRISPR/Cas technology

Nat Commun. 2014 Apr 24:5:3728. doi: 10.1038/ncomms4728.

Authors

Peter S Choi¹, Matthew Meyerson²

Affiliations

¹ 1] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA.
² 1] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA [3] Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

Targeted genomic rearrangements using CRISPR/Cas technology

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