Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy

Robert Prevedel; Young-Gyu Yoon; Maximilian Hoffmann; Nikita Pak; Gordon Wetzstein; Saul Kato; Tina Schrödel; Ramesh Raskar; Manuel Zimmer; Edward S Boyden; Alipasha Vaziri

doi:10.1038/nmeth.2964

Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy

Nat Methods. 2014 Jul;11(7):727-730. doi: 10.1038/nmeth.2964. Epub 2014 May 18.

Authors

Robert Prevedel^#^{1

2

3}, Young-Gyu Yoon^#^{4

5}, Maximilian Hoffmann^{1

2

3}, Nikita Pak^{5

6}, Gordon Wetzstein⁵, Saul Kato¹, Tina Schrödel¹, Ramesh Raskar⁵, Manuel Zimmer¹, Edward S Boyden^{5

7

8

9}, Alipasha Vaziri^{1

2

3}

Affiliations

¹ Research Institute of Molecular Pathology, Vienna, Austria.
² Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
³ Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Austria.
⁴ Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
⁵ MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
⁶ Department of Mechanical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
⁷ Department of Biological Engineering, Massachusetts Institute of Technology(MIT), Cambridge, MA, USA.
⁸ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
⁹ McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.

^# Contributed equally.

Abstract

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Caenorhabditis elegans
Calcium / metabolism*
Calcium Signaling
Imaging, Three-Dimensional / methods*
Larva / ultrastructure
Microscopy / methods*
Microscopy, Fluorescence / methods
Neurons / physiology*
Zebrafish

Substances

Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding