Abstract
Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Axons / physiology*
-
DNA / administration & dosage
-
DNA / genetics
-
Electroporation / economics
-
Electroporation / methods*
-
Female
-
Ganglia, Spinal / physiology
-
Ganglia, Spinal / surgery
-
Mice
-
Microinjections / methods
-
Microscopy, Fluorescence / methods
-
Nerve Regeneration*
-
Neurosurgical Procedures / methods
-
Oligoribonucleotides, Antisense / administration & dosage
-
Plasmids / administration & dosage*
-
Plasmids / genetics
-
Sciatic Nerve / physiology
-
Sciatic Nerve / surgery
-
Sensory Receptor Cells / physiology*
-
Transfection / economics
-
Transfection / methods*
Substances
-
Oligoribonucleotides, Antisense
-
DNA