Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP)

Xuzhi Zhang; Stuart B Lowe; John Justin Gooding

doi:10.1016/j.bios.2014.05.039

Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP)

Biosens Bioelectron. 2014 Nov 15:61:491-9. doi: 10.1016/j.bios.2014.05.039. Epub 2014 Jun 3.

Authors

Xuzhi Zhang¹, Stuart B Lowe², John Justin Gooding³

Affiliations

¹ Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, PR China. Electronic address: zhangxz@ysfri.ac.cn.
² School of Chemistry and Australian Centre for NanoMedicine, The University of New South Wales, Sydney, NSW 2052, Australia.
³ School of Chemistry and Australian Centre for NanoMedicine, The University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: justin.gooding@unsw.edu.au.

PMID: 24949822
DOI: 10.1016/j.bios.2014.05.039

Abstract

The loop-mediated isothermal amplification (LAMP) technique has the potential to revolutionize molecular biology because it allows DNA amplification under isothermal conditions and is highly compatible with point-of-care analysis. To achieve efficient genetic analysis of samples, the method of real-time or endpoint determination selected to monitor the biochemical reaction is of great importance. In this paper we briefly review progress in the development of monitoring methods for LAMP.

Keywords: Biosensor; LAMP; Loop-mediated isothermal amplification; Monitoring methods; Point-of-care analysis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Humans
Nucleic Acid Amplification Techniques / instrumentation
Nucleic Acid Amplification Techniques / methods*
Point-of-Care Systems
Temperature