Abstract
Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3-Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a=51.6, b=86.9, c=88.7 Å.
Keywords:
90S pre-ribosome; Imp3; Mpp10; Saccharomyces cerevisiae; ribosome synthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Molecular Sequence Data
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Molecular Weight
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Peptide Fragments / chemistry*
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Peptide Fragments / genetics
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Peptide Fragments / metabolism
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Phosphoproteins / chemistry*
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Protein Binding
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribonucleoproteins / chemistry*
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Ribonucleoproteins / genetics
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Ribonucleoproteins / metabolism
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Ribosomal Proteins / chemistry*
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Ribosomes / chemistry
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Ribosomes / metabolism
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Saccharomyces cerevisiae / chemistry*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
Substances
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IMP3 protein, S cerevisiae
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MPP10 protein, S cerevisiae
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Peptide Fragments
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Phosphoproteins
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Recombinant Fusion Proteins
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Ribonucleoproteins
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins