By the six-cell stage, embryos of Caenorhabditis elegans are morphologically L–R asymmetric with an invariant handedness that persists throughout development. We used intracellular markers to ask whether breaking of L–R symmetry could be observed at earlier stages. Observation of two- to three-cell embryos carrying intracellular markers indicated that L–R symmetry is broken concomitantly with establishment of D–V axis polarity during division of the anterior AB cell. The AB cleavage furrow initiates asymmetrically and always from the left, suggesting L–R differences in the AB cell cortex. An invariantly handed cortical rotation observed earlier during first cleavage implies that the one-cell embryo has an intrinsic chirality. We propose that L–R differences in the cortex could result from mechanical forces on asymmetric components of a chiral cortical network during the off-axis elongation of the AB-cell spindle prior to AB cleavage.