Abstract
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
DNA-Directed DNA Polymerase / metabolism*
-
Fluorescence Resonance Energy Transfer / methods*
-
Humans
-
Polyethylene Glycols / chemistry*
-
Polyethylene Glycols / metabolism
-
Polysorbates / chemistry*
-
Polysorbates / metabolism
-
Proliferating Cell Nuclear Antigen / metabolism*
-
Silanes / chemistry*
-
Silanes / metabolism
-
Surface Properties
Substances
-
Polysorbates
-
Proliferating Cell Nuclear Antigen
-
Silanes
-
Polyethylene Glycols
-
dichlorodimethylsilane
-
DNA-Directed DNA Polymerase
-
POLK protein, human