Abstract
Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Blotting, Southern
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Drug Resistance, Microbial / genetics
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Genes, Bacterial
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Genes, Fungal
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Genetic Markers
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Neurospora / genetics*
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Neurospora crassa / drug effects
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Neurospora crassa / genetics*
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Organophosphorus Compounds / pharmacology*
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Plasmids
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Promoter Regions, Genetic
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Restriction Mapping
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Streptomyces / genetics
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Transformation, Genetic*
Substances
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Anti-Bacterial Agents
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Genetic Markers
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Organophosphorus Compounds
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bialaphos