Abstract
Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Extracellular Matrix Proteins / genetics
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Fluorescent Dyes / chemistry*
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Gene Knock-In Techniques / methods*
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Genes, Reporter*
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Integrases* / genetics
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Mice, Transgenic
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O(6)-Methylguanine-DNA Methyltransferase / chemistry
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O(6)-Methylguanine-DNA Methyltransferase / genetics
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Protein-Lysine 6-Oxidase / genetics
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RNA, Untranslated / genetics
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / genetics
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Staining and Labeling
Substances
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Extracellular Matrix Proteins
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Fluorescent Dyes
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Gt(ROSA)26Sor non-coding RNA, mouse
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RNA, Untranslated
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Recombinant Fusion Proteins
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Lox protein, mouse
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Protein-Lysine 6-Oxidase
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O(6)-Methylguanine-DNA Methyltransferase
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Cre recombinase
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Integrases