Abstract
Sub1 and Maf1 exert an opposite effect on RNA polymerase III transcription interfering with different steps of the transcription cycle. In this study, we present evidence that Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span. First, cells lacking Sub1 need more time than wild type to exit from resting and this lag in re-proliferation is correlated with a delay in transcriptional reactivation. Second, our data show that the capacity of the cells to properly establish a quiescent state is impaired in the absence of Sub1 resulting in a premature death that is dependent on the Ras/PKA and Tor1/Sch9 signalling pathways. On the other hand, we show that maf1Δ cells are long-lived mutant suggesting a connection between Pol III transcription and yeast longevity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle*
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Cell Proliferation
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Cell Survival
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Cyclic AMP-Dependent Protein Kinases / metabolism
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DNA-Binding Proteins / metabolism*
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Longevity
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Phosphatidylinositol 3-Kinases / metabolism
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Protein Serine-Threonine Kinases / metabolism
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RNA Polymerase III / metabolism*
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Reactive Oxygen Species / metabolism
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins / metabolism*
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Signal Transduction
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Transcription Factors / metabolism*
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Transcriptional Activation
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ras Proteins / metabolism
Substances
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DNA-Binding Proteins
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MAF1 protein, S cerevisiae
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Reactive Oxygen Species
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SUB1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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TOR1 protein, S cerevisiae
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Protein Serine-Threonine Kinases
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SCH9 protein, S cerevisiae
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Cyclic AMP-Dependent Protein Kinases
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RNA Polymerase III
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ras Proteins
Grants and funding
This work was supported by the French National Research Agency (grant 2010-BLAN-1605-01) and the International PhD Program of the CEA to AT and NTTN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.