Abstract
The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Behavior, Animal
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Drosophila Proteins / genetics
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Embryo, Nonmammalian
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Female
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Gene Expression Regulation
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Genetic Engineering / methods*
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Green Fluorescent Proteins / genetics
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Larva
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Male
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Promoter Regions, Genetic
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Sleep / genetics
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Trans-Activators / genetics*
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Transcription Factors / genetics
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Transgenes*
Substances
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Drosophila Proteins
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GAL4 protein, Drosophila
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Trans-Activators
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Transcription Factors
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Green Fluorescent Proteins