Abstract
To examine the interaction between molecular, electrical and behavioral circadian rhythms, we combined optogenetic manipulation of suprachiasmatic nucleus (SCN) firing rate with bioluminescence imaging and locomotor activity monitoring. Manipulating firing rate reset circadian rhythms both ex vivo and in vivo, and this resetting required spikes and network communication. This suggests that SCN firing rate is fundamental to circadian pacemaking as both an input to and output of the molecular clockworks.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Action Potentials / drug effects
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Action Potentials / physiology*
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Animals
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Channelrhodopsins
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Circadian Rhythm / physiology*
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In Vitro Techniques
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Motor Activity / genetics
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Motor Activity / physiology*
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Neurons / drug effects
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Neurons / physiology*
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Patch-Clamp Techniques
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Period Circadian Proteins / genetics
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Period Circadian Proteins / metabolism
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Photic Stimulation
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Receptors, Dopamine D1 / genetics
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Receptors, Dopamine D1 / metabolism
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Sodium Channel Blockers / pharmacology
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Suprachiasmatic Nucleus / cytology*
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Tetrodotoxin / pharmacology
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Time Factors
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Channelrhodopsins
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Drd1 protein, mouse
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Luminescent Proteins
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Per2 protein, mouse
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Period Circadian Proteins
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Receptors, Dopamine D1
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Sodium Channel Blockers
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vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-
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Vasoactive Intestinal Peptide
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Tetrodotoxin