Arabidopsis HFR1 is a potential nuclear substrate regulated by the Xanthomonas type III effector XopD(Xcc8004)

Choon Meng Tan; Meng-Ying Li; Pei-Yun Yang; Shu Heng Chang; Yi-Ping Ho; Hong Lin; Wen-Ling Deng; Jun-Yi Yang

doi:10.1371/journal.pone.0117067

Arabidopsis HFR1 is a potential nuclear substrate regulated by the Xanthomonas type III effector XopD(Xcc8004)

PLoS One. 2015 Feb 3;10(2):e0117067. doi: 10.1371/journal.pone.0117067. eCollection 2015.

Authors

Choon Meng Tan¹, Meng-Ying Li², Pei-Yun Yang², Shu Heng Chang², Yi-Ping Ho², Hong Lin², Wen-Ling Deng³, Jun-Yi Yang⁴

Affiliations

¹ Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan.
² Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
³ Department of Plant Pathology, National ChungHsing University, Taichung, Taiwan.
⁴ Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan; Institute of Biotechnology, National ChungHsing University, Taichung, Taiwan; NCHU-UCD Plant and Food Biotechnology Center, National ChungHsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National ChungHsing University, Taichung, Taiwan.

Abstract

XopDXcc8004, a type III effector of Xanthomonas campestris pv. campestris (Xcc) 8004, is considered a shorter version of the XopD, which lacks the N-terminal domain. To understand the functions of XopDXcc8004, in planta, a transgenic approach combined with inducible promoter to analyze the effects of XopDXcc8004 in Arabidopsis was done. Here, the expression of XopDXcc8004, in Arabidopsis elicited the accumulation of host defense-response genes. These molecular changes were dependent on salicylic acid and correlated with lesion-mimic phenotypes observed in XVE::XopDXcc8004 transgenic plants. Moreover, XopDXcc8004 was able to desumoylate HFR1, a basic helix-loop-helix transcription factor involved in photomorphogenesis, through SUMO protease activity. Interestingly, the hfr1-201 mutant increased the expression of host defense-response genes and displayed a resistance phenotype to Xcc8004. These data suggest that HFR1 is involved in plant innate immunity and is potentially regulated by XopDXcc8004.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics*
Arabidopsis / immunology
Arabidopsis / microbiology
Arabidopsis Proteins / genetics
Arabidopsis Proteins / immunology*
Bacterial Proteins / genetics*
Bacterial Proteins / immunology
DNA-Binding Proteins / genetics
DNA-Binding Proteins / immunology*
Gene Expression Regulation, Plant*
Host-Pathogen Interactions*
Mutation
Nuclear Proteins / genetics
Nuclear Proteins / immunology*
Plant Diseases / genetics*
Plant Diseases / immunology
Plant Diseases / microbiology
Plants, Genetically Modified / genetics
Plants, Genetically Modified / immunology
Salicylic Acid / immunology
Small Ubiquitin-Related Modifier Proteins / immunology
Xanthomonas campestris / physiology*

Substances

Arabidopsis Proteins
Bacterial Proteins
DNA-Binding Proteins
HFR1 protein, Arabidopsis
Nuclear Proteins
Small Ubiquitin-Related Modifier Proteins
Salicylic Acid

Grants and funding

This work was supported by the Ministry of Science and Technology (NSC 100-2321-B- 005-007-MY3, NSC 103-2911-I-005-301, MOST 103-2321-B-005-020) and Ministry of Education (ATU plan), Taiwan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.