DIP1 plays an antiviral role against DCV infection in Drosophila melanogaster

Qi Zhang; Liqin Zhang; Xinlei Gao; Shuishui Qi; Zhaoxia Chang; Qingfa Wu

doi:10.1016/j.bbrc.2015.03.013

DIP1 plays an antiviral role against DCV infection in Drosophila melanogaster

Biochem Biophys Res Commun. 2015 May 1;460(2):222-6. doi: 10.1016/j.bbrc.2015.03.013. Epub 2015 Mar 11.

Authors

Qi Zhang¹, Liqin Zhang¹, Xinlei Gao¹, Shuishui Qi¹, Zhaoxia Chang¹, Qingfa Wu²

Affiliations

¹ School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
² School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China; The CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China, Hefei, Anhui 230027, China. Electronic address: wuqf@ustc.edu.cn.

PMID: 25770426
DOI: 10.1016/j.bbrc.2015.03.013

Abstract

Disconnected Interacting Protein 1 (DIP1) is a dsRNA-binding protein that participates in a wide range of cellular processes. Whether DIP1 is involved in innate immunity remains unclear. Here, DIP1 was found to play an antiviral role in S2 cells. Its antiviral action is specific for DCV infection and not for DXV infection. dip1 mutant flies are hypersensitive to DCV infection. The increased mortality in dip1 mutant flies is associated with the accumulation of DCV positive-stranded RNAs in vivo. This study demonstrated that dip1 is a novel antiviral gene that restricts DCV replication in vitro and in vivo.

Keywords: Antiviral gene; DCV; DIP1; dsRNA binding domain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
DNA Primers
Drosophila Proteins / physiology*
Drosophila melanogaster
Insect Viruses / pathogenicity*
Transcription Factors / physiology*
Virus Diseases / physiopathology
Virus Diseases / prevention & control

Substances

DISCO Interacting Protein 1, Drosophila
DNA Primers
Drosophila Proteins
Transcription Factors