Abstract
Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Caspase 9 / drug effects
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Caspase 9 / metabolism*
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Cells, Cultured
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Embryonic Stem Cells / metabolism
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Enhancer Elements, Genetic / genetics
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Enhancer Elements, Genetic / physiology*
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Gene Expression Regulation, Enzymologic
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Genes, Reporter
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Histone Demethylases / drug effects
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Histone Demethylases / metabolism*
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Histones / metabolism
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Mice
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Neisseria meningitidis / enzymology
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Recombinant Proteins / metabolism
Substances
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Bacterial Proteins
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Histones
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Recombinant Proteins
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Histone Demethylases
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Caspase 9