Abstract
Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Chromobacterium / enzymology
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DNA, Bacterial
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Escherichia coli / metabolism*
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Gene Expression Regulation, Bacterial / physiology*
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Genes, Reporter
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Luminescent Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Red Fluorescent Protein
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Riboswitch
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Vibrio / enzymology*
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Vibrio / genetics
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Vibrio / metabolism
Substances
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Bacterial Proteins
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DNA, Bacterial
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Luminescent Proteins
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RNA, Messenger
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Recombinant Proteins
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Riboswitch