Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration

Yoshihiro Nakamura; Shigeru Miyaki; Hiroyuki Ishitobi; Sho Matsuyama; Tomoyuki Nakasa; Naosuke Kamei; Takayuki Akimoto; Yukihito Higashi; Mitsuo Ochi

doi:10.1016/j.febslet.2015.03.031

Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration

FEBS Lett. 2015 May 8;589(11):1257-65. doi: 10.1016/j.febslet.2015.03.031. Epub 2015 Apr 8.

Authors

Yoshihiro Nakamura¹, Shigeru Miyaki², Hiroyuki Ishitobi³, Sho Matsuyama⁴, Tomoyuki Nakasa⁴, Naosuke Kamei², Takayuki Akimoto⁵, Yukihito Higashi³, Mitsuo Ochi⁴

Affiliations

¹ Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan. Electronic address: nakamurayoshihiro0419@gmail.com.
² Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan; Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
³ Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
⁴ Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan.
⁵ Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.

PMID: 25862500
DOI: 10.1016/j.febslet.2015.03.031

Abstract

Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regeneration in an in vivo model of muscle injury. Although MSC exosomes had low concentrations of muscle-repair-related cytokines, a number of repair-related miRNAs were identified. This study suggests that the MSC-derived exosomes promote muscle regeneration by enhancing myogenesis and angiogenesis, which is at least in part mediated by miRNAs such as miR-494.

Keywords: Exosome; Mesenchymal stem cell; MicroRNA; Regeneration; Skeletal muscle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Exosomes*
Humans
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism*
Mice
MicroRNAs / biosynthesis
Muscle Development
Muscle, Skeletal / cytology
Muscle, Skeletal / physiology*
Myoblasts, Skeletal / cytology
Myoblasts, Skeletal / metabolism*
Regeneration / physiology*

Substances

MicroRNAs
Mirn494 microRNA, mouse