A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

ACS Synth Biol. 2015 Sep 18;4(9):975-86. doi: 10.1021/sb500366v. Epub 2015 May 1.

Abstract

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of its long history in industrial fermentations. However, until recently, most synthetic biology systems have focused on bacteria. While there is a wealth of resources and literature about the biology of yeast, it can be daunting to navigate and extract the tools needed for engineering applications. Here we present a versatile engineering platform for yeast, which contains both a rapid, modular assembly method and a basic set of characterized parts. This platform provides a framework in which to create new designs, as well as data on promoters, terminators, degradation tags, and copy number to inform those designs. Additionally, we describe genome-editing tools for making modifications directly to the yeast chromosomes, which we find preferable to plasmids due to reduced variability in expression. With this toolkit, we strive to simplify the process of engineering yeast by standardizing the physical manipulations and suggesting best practices that together will enable more straightforward translation of materials and data from one group to another. Additionally, by relieving researchers of the burden of technical details, they can focus on higher-level aspects of experimental design.

Keywords: MoClo; characterized parts; golden gate; toolkit; yeast.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA Copy Number Variations
  • DNA, Fungal / genetics
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Fungal*
  • Genetic Engineering / methods*
  • Multigene Family
  • Plasmids / genetics
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Transcriptional Activation

Substances

  • DNA, Fungal