Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

Mol Imaging Biol. 2015 Dec;17(6):848-55. doi: 10.1007/s11307-015-0858-0.

Abstract

Purpose: The current study presents [(18)F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging.

Procedures: [(18)F]PARPi-FL was obtained by (19)F/(18)F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model.

Results: [(18)F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [(18)F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography.

Conclusions: The fluorescent component of [(18)F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [(18)F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.

Keywords: Fluorescence; Glioblastoma; Imaging; Multimodality; PARP1; PET; U87 MG.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / enzymology
  • Brain Neoplasms / enzymology*
  • Brain Neoplasms / pathology
  • Case-Control Studies
  • Fluorescence
  • Glioblastoma / enzymology*
  • Glioblastoma / pathology
  • Half-Life
  • Heterografts
  • Humans
  • Mice
  • Multimodal Imaging*
  • Optical Imaging*
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases / metabolism*
  • Positron-Emission Tomography*

Substances

  • Parp1 protein, mouse
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases