Off-target mutations are rare in Cas9-modified mice

Nat Methods. 2015 Jun;12(6):479. doi: 10.1038/nmeth.3408.

Authors

Vivek Iyer¹, Bin Shen², Wensheng Zhang³, Alex Hodgkins¹, Thomas Keane¹, Xingxu Huang², William C Skarnes¹

Affiliations

¹ Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
² Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
³ 1] Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK. [2] Cambridge-Suda Genome Research Center, Soochow University, Suzhou, China.

PMID: 26020497
DOI: 10.1038/nmeth.3408

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breeding
CRISPR-Associated Proteins / genetics
CRISPR-Associated Proteins / metabolism*
Clustered Regularly Interspaced Short Palindromic Repeats*
Deoxyribonuclease I / genetics
Deoxyribonuclease I / metabolism
Female
Gene Expression Regulation, Enzymologic
Genome
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mutation
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment

Substances

CRISPR-Associated Proteins
RNA, Messenger
Deoxyribonuclease I

Grants and funding