Traction microscopy to identify force modulation in subresolution adhesions

Nat Methods. 2015 Jul;12(7):653-6. doi: 10.1038/nmeth.3430. Epub 2015 Jun 1.

Abstract

We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Adhesion*
  • Cells, Cultured
  • Microscopy, Fluorescence / methods*
  • Software