Decoding enhancers using massively parallel reporter assays

Genomics. 2015 Sep;106(3):159-164. doi: 10.1016/j.ygeno.2015.06.005. Epub 2015 Jun 10.

Abstract

Enhancers control the timing, location and expression levels of their target genes. Nucleotide variation in enhancers has been shown to lead to numerous phenotypes, including human disease. While putative enhancer sequences and nucleotide variation within them can now be detected in a rapid manner using various genomic technologies, the understanding of the functional consequences of these variants still remains largely unknown. Massively parallel reporter assays (MPRAs) can overcome this hurdle by providing the ability to test thousands of sequences and nucleotide variants within them for enhancer activity en masse. Here, we describe this technology and specifically focus on how it is being used to obtain an increased understanding of enhancer regulatory code and grammar.

Keywords: Enhancer; Massively parallel reporter assays; Transcriptional regulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Binding Sites / genetics
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation
  • Genomics
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Promoter Regions, Genetic
  • Sequence Analysis, DNA / methods*
  • Transcription Factors / genetics*

Substances

  • Transcription Factors