Abstract
In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Algorithms
-
Animals
-
Brain / metabolism
-
Brain / pathology
-
Calcium / chemistry
-
Calibration
-
Cerebral Cortex / metabolism
-
Dendritic Cells / cytology
-
Green Fluorescent Proteins / chemistry
-
Image Processing, Computer-Assisted
-
Immune System
-
Lasers
-
Lymph Nodes / pathology
-
Mice
-
Mice, Transgenic
-
Microglia / metabolism
-
Microscopy, Fluorescence / methods*
-
Microscopy, Fluorescence, Multiphoton / methods*
-
Neurosciences
-
Neutrophils / cytology
-
Optics and Photonics
-
Oscillometry
Substances
-
Green Fluorescent Proteins
-
Calcium