Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy

Giuliano Scarcelli; William J Polacheck; Hadi T Nia; Kripa Patel; Alan J Grodzinsky; Roger D Kamm; Seok Hyun Yun

doi:10.1038/nmeth.3616

Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy

Nat Methods. 2015 Dec;12(12):1132-4. doi: 10.1038/nmeth.3616. Epub 2015 Oct 5.

Authors

Giuliano Scarcelli^{1

2

3}, William J Polacheck⁴, Hadi T Nia⁴, Kripa Patel¹, Alan J Grodzinsky^{4

5

6}, Roger D Kamm^{4

5}, Seok Hyun Yun^{1

2

7}

Affiliations

¹ Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
² Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
³ Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
⁴ Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
⁵ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
⁶ Department of Electrical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
⁷ The Harvard-MIT Health Sciences and Technology Program, Cambridge, Massachusetts, USA.

Abstract

Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Biomechanical Phenomena
Cell Size
Cytoskeleton / chemistry*
Cytoskeleton / ultrastructure
Elastic Modulus
Extracellular Matrix / chemistry*
Extracellular Matrix / ultrastructure
Imaging, Three-Dimensional
Mice
Microscopy, Confocal / instrumentation
Microscopy, Confocal / methods*
NIH 3T3 Cells
Osmotic Pressure

Abstract

Publication types

MeSH terms

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