Resources for the design of CRISPR gene editing experiments

Daniel B Graham; David E Root

doi:10.1186/s13059-015-0823-x

Resources for the design of CRISPR gene editing experiments

Genome Biol. 2015 Nov 27:16:260. doi: 10.1186/s13059-015-0823-x.

Authors

Daniel B Graham^{1

2

3}, David E Root⁴

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
² Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
³ Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, 02114, USA.
⁴ Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA. droot@broadinstitute.org.

Abstract

CRISPR-based approaches have quickly become a favored method to perturb genes to uncover their functions. Here, we review the key considerations in the design of genome editing experiments, and survey the tools and resources currently available to assist users of this technology.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacterial Proteins / genetics*
CRISPR-Associated Protein 9
CRISPR-Cas Systems / genetics*
Endonucleases / genetics*
Escherichia coli / genetics
Gene Expression Regulation
Gene Targeting
RNA Editing / genetics*
RNA, Guide, CRISPR-Cas Systems / genetics

Substances

Bacterial Proteins
RNA, Guide, CRISPR-Cas Systems
CRISPR-Associated Protein 9
Cas9 endonuclease Streptococcus pyogenes
Endonucleases