Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius

Jeffrey A Rosenfeld; Darryl Reeves; Mercer R Brugler; Apurva Narechania; Sabrina Simon; Russell Durrett; Jonathan Foox; Kevin Shianna; Michael C Schatz; Jorge Gandara; Ebrahim Afshinnekoo; Ernest T Lam; Alex R Hastie; Saki Chan; Han Cao; Michael Saghbini; Alex Kentsis; Paul J Planet; Vladyslav Kholodovych; Michael Tessler; Richard Baker; Rob DeSalle; Louis N Sorkin; Sergios-Orestis Kolokotronis; Mark E Siddall; George Amato; Christopher E Mason

doi:10.1038/ncomms10164

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius

Nat Commun. 2016 Feb 2:7:10164. doi: 10.1038/ncomms10164.

Authors

Jeffrey A Rosenfeld^{1

2

3}, Darryl Reeves^{4

5

6}, Mercer R Brugler^{1

2

7}, Apurva Narechania^{1

2}, Sabrina Simon^{1

8}, Russell Durrett^{4

5}, Jonathan Foox^{1

2}, Kevin Shianna⁹, Michael C Schatz¹⁰, Jorge Gandara^{4

5}, Ebrahim Afshinnekoo^{4

5}, Ernest T Lam¹¹, Alex R Hastie¹¹, Saki Chan¹¹, Han Cao¹¹, Michael Saghbini¹¹, Alex Kentsis^{12

13}, Paul J Planet^{1

2

14}, Vladyslav Kholodovych¹⁵, Michael Tessler^{1

2}, Richard Baker^{1

2}, Rob DeSalle^{1

2}, Louis N Sorkin², Sergios-Orestis Kolokotronis^{1

2

16}, Mark E Siddall^{1

2}, George Amato^{1

2}, Christopher E Mason^{4

5

6

17}

Affiliations

¹ Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, New York 10024, USA.
² Division of Invertebrate Zoology, American Museum of Natural History, New York, New York 10024, USA.
³ Cancer Institute of New Jersey, Rutgers University, New Brunswick, New Jersey 08908, USA.
⁴ Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York 10065, USA.
⁵ The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, New York, New York 10065, USA.
⁶ Tri-Institutional Training Program in Computational Biology and Medicine, New York, New York 10065, USA.
⁷ Biological Sciences Department, NYC College of Technology (CUNY), Brooklyn, New York 11201, USA.
⁸ Biosystematics, Wageningen University, Wageningen 6708 PB, The Netherlands.
⁹ Illumina Inc. 5200 Illumina Way, San Diego, California 92122, USA.
¹⁰ Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
¹¹ BioNanoGenomics Inc. 9640 Towne Centre Drive Ste. 100, San Diego, California 92121, USA.
¹² Molecular Pharmacology and Chemistry Program, Department of Pediatrics, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA.
¹³ Department of Pediatrics, Memorial Sloan Kettering Cancer Center, Weill Cornell Medical College, Cornell University, New York, New York 10065, USA.
¹⁴ Division of Pediatric Infectious Diseases, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
¹⁵ High Performance and Research Computing Group, Rutgers Biomedical and Health Sciences, Newark, New Jersey 07103, USA.
¹⁶ Department of Biological Sciences, Fordham University, Bronx, New York 10458, USA.
¹⁷ The Feil Family Brain and Mind Research Institute, New York, New York 10065, USA.

Abstract

The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bedbugs / genetics*
Blood
Chromosome Mapping
Eating
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Humans
Life Cycle Stages / genetics*
Male
Models, Molecular
Phylogeny
Sequence Analysis, RNA

Abstract

Publication types

MeSH terms

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