Abstract
Neuronal circuit function is governed by precise patterns of connectivity between specialized groups of neurons. The diversity of GABAergic interneurons is a hallmark of cortical circuits, yet little is known about their targeting to individual postsynaptic dendrites. We examined synaptic connectivity between molecularly defined inhibitory interneurons and CA1 pyramidal cell dendrites using correlative light-electron microscopy and large-volume array tomography. We show that interneurons can be highly selective in their connectivity to specific dendritic branch types and, furthermore, exhibit precisely targeted connectivity to the origin or end of individual branches. Computational simulations indicate that the observed subcellular targeting enables control over the nonlinear integration of synaptic input or the initiation and backpropagation of action potentials in a branch-selective manner. Our results demonstrate that connectivity between interneurons and pyramidal cell dendrites is more precise and spatially segregated than previously appreciated, which may be a critical determinant of how inhibition shapes dendritic computation.
Copyright © 2016 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
-
Video-Audio Media
MeSH terms
-
Action Potentials / drug effects
-
Action Potentials / genetics
-
Action Potentials / physiology
-
Animals
-
CA1 Region, Hippocampal / cytology*
-
Computer Simulation
-
Dendrites / physiology*
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
Male
-
Mice
-
Mice, Transgenic
-
Microscopy, Electron, Transmission
-
Models, Neurological
-
Neural Inhibition / drug effects
-
Neural Inhibition / genetics
-
Neural Inhibition / physiology*
-
Neurons / cytology*
-
Neurons / physiology*
-
Neuropeptide Y / genetics
-
Neuropeptide Y / metabolism
-
Neuropeptide Y / pharmacology
-
Nitric Oxide Synthase Type I / genetics
-
Nitric Oxide Synthase Type I / metabolism
-
Parvalbumins / genetics
-
Parvalbumins / metabolism
-
Somatostatin / genetics
-
Somatostatin / metabolism
-
Synapses / metabolism
-
Synapses / physiology
-
Synapses / ultrastructure
-
Vesicular Inhibitory Amino Acid Transport Proteins / genetics
-
Vesicular Inhibitory Amino Acid Transport Proteins / metabolism
Substances
-
Neuropeptide Y
-
Parvalbumins
-
Vesicular Inhibitory Amino Acid Transport Proteins
-
Viaat protein, mouse
-
enhanced green fluorescent protein
-
Green Fluorescent Proteins
-
Somatostatin
-
Nitric Oxide Synthase Type I