Expansion microscopy with conventional antibodies and fluorescent proteins

Tyler J Chozinski; Aaron R Halpern; Haruhisa Okawa; Hyeon-Jin Kim; Grant J Tremel; Rachel O L Wong; Joshua C Vaughan

doi:10.1038/nmeth.3833

Expansion microscopy with conventional antibodies and fluorescent proteins

Nat Methods. 2016 Jun;13(6):485-8. doi: 10.1038/nmeth.3833. Epub 2016 Apr 11.

Authors

Tyler J Chozinski¹, Aaron R Halpern¹, Haruhisa Okawa², Hyeon-Jin Kim¹, Grant J Tremel¹, Rachel O L Wong², Joshua C Vaughan^{1

3}

Affiliations

¹ Department of Chemistry, University of Washington, Seattle, Washington, USA.
² Department of Biological Structure, University of Washington, Seattle, Washington, USA.
³ Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA.

Abstract

Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Animals
Antibodies, Monoclonal*
Brain / ultrastructure
Cell Line
Image Enhancement / methods*
Luminescent Proteins* / genetics
Mice, Inbred C57BL
Microscopy, Confocal / methods*
Microscopy, Fluorescence / methods*
Reproducibility of Results
Sensitivity and Specificity
Staining and Labeling
Transfection

Substances

Antibodies, Monoclonal
Luminescent Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding