Direct Electron Detectors

Methods Enzymol. 2016:579:1-17. doi: 10.1016/bs.mie.2016.05.056. Epub 2016 Jul 5.

Abstract

Direct electron detectors have played a key role in the recent increase in the power of single-particle electron cryomicroscopy (cryoEM). In this chapter, we summarize the background to these recent developments, give a practical guide to their optimal use, and discuss future directions.

Keywords: CMOS; DDD; DQE; Direct Electron; Electron microscopy; FEI; Gatan; MTF; Sensor; cryoEM.

Publication types

  • Historical Article
  • Review

MeSH terms

  • Bacterial Proteins / ultrastructure
  • Biosensing Techniques / history
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Cryoelectron Microscopy / history
  • Cryoelectron Microscopy / instrumentation
  • Cryoelectron Microscopy / methods*
  • Electrons*
  • Escherichia coli / chemistry
  • Escherichia coli / enzymology
  • History, 20th Century
  • History, 21st Century
  • Monte Carlo Method
  • Silicon / chemistry
  • Software*
  • beta-Galactosidase / ultrastructure

Substances

  • Bacterial Proteins
  • beta-Galactosidase
  • Silicon