Abstract
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.
MeSH terms
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Animals
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B-Lymphocytes / metabolism
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Cell Cycle Proteins / chemistry*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Cell Line
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Cell Nucleus / metabolism
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Chromatin / chemistry*
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Chromatin / genetics
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Chromatin / metabolism
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Chromatin Immunoprecipitation / methods*
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Chromosomal Proteins, Non-Histone / chemistry*
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism
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Chromosome Mapping
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Cohesins
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DNA / chemistry*
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Embryonic Stem Cells / metabolism
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Genomics / methods*
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Humans
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Mice
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA