Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

Utpal Bose; Catharine A Ortori; Sarir Sarmad; David A Barrett; Amitha K Hewavitharana; Mark P Hodson; John A Fuerst; P Nicholas Shaw; Rich Boden

doi:10.1093/femsle/fnx002

Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

FEMS Microbiol Lett. 2017 Jan 1;364(2). doi: 10.1093/femsle/fnx002.

Authors

Utpal Bose¹, Catharine A Ortori², Sarir Sarmad², David A Barrett², Amitha K Hewavitharana¹, Mark P Hodson^{1

3}, John A Fuerst⁴, P Nicholas Shaw¹, Rich Boden

Affiliations

¹ School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
² Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
³ Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
⁴ School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

PMID: 28087611
DOI: 10.1093/femsle/fnx002

Abstract

The structures of acyl homoserine lactone (AHL) compounds and their quantification were accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone, were characterised and quantified in Salinispora sp. cultures.

Keywords: homoserine lactone; liquid chromatography; marine bacteria; mass spectrometry.

MeSH terms

Acyl-Butyrolactones / analysis*
Animals
Aquatic Organisms / chemistry
Aquatic Organisms / isolation & purification
Aquatic Organisms / metabolism*
Chromatography, Liquid
Culture Media / chemistry
Mass Spectrometry
Micromonosporaceae / chemistry
Micromonosporaceae / isolation & purification
Micromonosporaceae / metabolism*
Porifera / microbiology*

Substances

Acyl-Butyrolactones
Culture Media