Abstract
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Pairing / genetics
-
CRISPR-Associated Proteins / genetics*
-
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
-
Cytidine Deaminase / genetics
-
DNA, Plant / genetics*
-
Gene Editing / methods*
-
Genes, Plant / genetics
-
Mutagenesis, Site-Directed / methods*
-
Oryza / genetics
-
Plants / genetics*
-
Plants, Genetically Modified / genetics
-
Point Mutation / genetics
-
Recombinant Fusion Proteins / genetics
-
Triticum / genetics
-
Zea mays / genetics
Substances
-
CRISPR-Associated Proteins
-
DNA, Plant
-
Recombinant Fusion Proteins
-
Cytidine Deaminase