A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM

Affiliations

• ¹ Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA; School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.
• ² Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
• ³ Department of Biochemistry and Molecular Biophysics, Columbia University New York, NY 10027, USA.
• ⁴ Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
• ⁵ Department of Biological Sciences, Barnard College, New York, NY 10027, USA.
• ⁶ Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
• ⁷ Department of Biochemistry and Molecular Biophysics, Columbia University New York, NY 10027, USA; Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA.
• ⁸ School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.
• ⁹ Department of Biochemistry and Molecular Biophysics, Columbia University New York, NY 10027, USA; Department of Biological Sciences, Columbia University, New York, NY 10027, USA; Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA. Electronic address: jf2192@cumc.columbia.edu.
• ¹⁰ Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA. Electronic address: qlin@columbia.edu.