Abstract
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pairing / genetics
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CRISPR-Associated Proteins / genetics*
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Clustered Regularly Interspaced Short Palindromic Repeats / genetics
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Cytidine Deaminase / genetics
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DNA, Plant / genetics*
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Gene Editing / methods*
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Genes, Plant / genetics
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Mutagenesis, Site-Directed / methods*
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Oryza / genetics*
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Plants, Genetically Modified / genetics
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Point Mutation / genetics
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Recombinant Fusion Proteins / genetics
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Solanum lycopersicum / genetics*
Substances
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CRISPR-Associated Proteins
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DNA, Plant
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Recombinant Fusion Proteins
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Cytidine Deaminase