Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus

M S Colett; R Larson; C Gold; D Strick; D K Anderson; A F Purchio

doi:10.1016/0042-6822(88)90672-1

Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus

Virology. 1988 Jul;165(1):191-9. doi: 10.1016/0042-6822(88)90672-1.

Authors

M S Colett¹, R Larson, C Gold, D Strick, D K Anderson, A F Purchio

Affiliation

¹ Molecular Genetics, Inc., Minnetonka, Minnesota 55343.

PMID: 2838957
DOI: 10.1016/0042-6822(88)90672-1

Abstract

The RNA genome of the cytopathic NADL isolate of bovine viral diarrhea virus (BVDV) has been molecularly cloned and the nucleotide sequence determined. The cloned sequence was 12,573 nucleotides in length, corresponding to a molecular weight of 4.3 X 10(6), having a base composition of 32.2% A, 25.7% G, 22.1% U, and 20.0% C. However, the sequences at the 5' and 3' termini of the RNA have not been unequivocally established. A single major open reading frame extending the length of the molecule was found in the viral-sense (positive polarity) sequence. This open reading frame was capable of encoding 3988 amino acids, representing 449 kDa of protein.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Base Sequence
Diarrhea Viruses, Bovine Viral / genetics*
Genes, Viral*
Molecular Sequence Data
Pestivirus / genetics*
RNA, Viral / genetics*

Substances

RNA, Viral

Associated data

GENBANK/UNKNOWN