Conformational Changes of CFTR upon Phosphorylation and ATP Binding

Cell. 2017 Jul 27;170(3):483-491.e8. doi: 10.1016/j.cell.2017.06.041. Epub 2017 Jul 20.

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel evolved from an ATP-binding cassette transporter. CFTR channel gating is strictly coupled to phosphorylation and ATP hydrolysis. Previously, we reported essentially identical structures of zebrafish and human CFTR in the dephosphorylated, ATP-free form. Here, we present the structure of zebrafish CFTR in the phosphorylated, ATP-bound conformation, determined by cryoelectron microscopy to 3.4 Å resolution. Comparison of the two conformations shows major structural rearrangements leading to channel opening. The phosphorylated regulatory domain is disengaged from its inhibitory position; the nucleotide-binding domains (NBDs) form a "head-to-tail" dimer upon binding ATP; and the cytoplasmic pathway, found closed off in other ATP-binding cassette transporters, is cracked open, consistent with CFTR's unique channel function. Unexpectedly, the extracellular mouth of the ion pore remains closed, indicating that local movements of the transmembrane helices can control ion access to the pore even in the NBD-dimerized conformation.

Keywords: ABC transporter; ATP-bound; CFTR; anion channel; cryo-EM; phosphorylated form.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Cryoelectron Microscopy
  • Cystic Fibrosis Transmembrane Conductance Regulator / chemistry*
  • Cystic Fibrosis Transmembrane Conductance Regulator / metabolism
  • Humans
  • Models, Molecular
  • Protein Domains
  • Sequence Alignment
  • Zebrafish Proteins / chemistry*
  • Zebrafish Proteins / metabolism

Substances

  • CFTR protein, zebrafish
  • Zebrafish Proteins
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Adenosine Triphosphate