Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA

Nature. 1986;323(6091):777-81. doi: 10.1038/323777a0.

Abstract

Processing of the 3' end of sea urchin H3 histone pre-mRNA requires conserved sequence elements and the presence of U7 snRNA. A mutation in the conserved CAAGAAGA sequence of the H3 pre-mRNA that renders 3' processing of this precursor defective is shown to be suppressed by a compensatory change in the U7 snRNA, restoring the base-pairing potential of the two RNAs. RNA-RNA contacts between these two molecules appear to be an essential feature of the 3' processing reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Restriction Enzymes / metabolism
  • Histones / genetics
  • Mutation*
  • Nucleic Acid Conformation
  • Nucleic Acid Hybridization
  • Nucleic Acid Precursors / analysis
  • Oocytes / analysis
  • RNA Precursors
  • RNA, Messenger / analysis*
  • RNA, Small Nuclear / analysis*
  • Sea Urchins
  • Xenopus

Substances

  • Histones
  • Nucleic Acid Precursors
  • RNA Precursors
  • RNA, Messenger
  • RNA, Small Nuclear
  • DNA Restriction Enzymes