Deletion analysis of GAL4 defines two transcriptional activating segments

Cell. 1987 Mar 13;48(5):847-53. doi: 10.1016/0092-8674(87)90081-x.

Abstract

We describe the activities of a wide array of deletion mutants of GAL4, a yeast transcriptional activator. We identify two short regions of GAL4, each of which activates transcription when fused to the DNA-binding region of the molecule. Very large portions of GAL4 are not required for gene activation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Deletion*
  • DNA Restriction Enzymes
  • Galactose / metabolism*
  • Mutation
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic*

Substances

  • DNA Restriction Enzymes
  • Galactose